MirGeneDB 2.1
MirGeneDB ID

Tni-Mir-27-P2b

Family name MIR-27 (all species)
Seed UCACAGU
Species Pufferfish (Tetraodon nigroviridis)
MiRBase ID tni-mir-27b
Paralogues Tni-Mir-27-P1  Tni-Mir-27-P3  Tni-Mir-27-P4a  Tni-Mir-27-P4b 
Orthologues Aca-Mir-27-P2  Ami-Mir-27-P2  Bta-Mir-27-P2  Cfa-Mir-27-P2  Cli-Mir-27-P2  Cmi-Mir-27-P2  Cpi-Mir-27-P2  Cpo-Mir-27-P2  Dno-Mir-27-P2  Dre-Mir-27-P2b  Ete-Mir-27-P2  Gga-Mir-27-P2  Gja-Mir-27-P2  Gmo-Mir-27-P2b  Hsa-Mir-27-P2  Lch-Mir-27-P2  Loc-Mir-27-P2  Mal-Mir-27-P2b  Mdo-Mir-27-P2  Mml-Mir-27-P2  Mmu-Mir-27-P2  Mun-Mir-27-P2  Oan-Mir-27-P2  Ocu-Mir-27-P2  Pbv-Mir-27-P2  Pma-Mir-27-o2  Rno-Mir-27-P2  Sha-Mir-27-P2  Spt-Mir-27-P2  Sto-Mir-27-P2  Tgu-Mir-27-P2  Xtr-Mir-27-P2 
Node of Origin (locus) Clupeocephala
Node of Origin (family) Vertebrata
Genome context
(Tetraodon_nigroviridis.TETRAODON8.dna.toplevel)		 12: 8516519-8516581 [+] UCSC Ensembl
Clustered miRNAs
(< 50kb from Mir-27-P2b)		 Mir-23-P2b 12: 8516226-8516284 [+] UCSC Ensembl
Mir-27-P2b 12: 8516519-8516581 [+] UCSC Ensembl
Mir-24-P2b 12: 8518120-8518178 [+] UCSC Ensembl
Precursor
(pre-Mir +30nt flank)
CUCUCUCUCUCUCUCUCUCCAGCCAGGCACAGAGCUUAGCUGAUUGGUGAACAGUGAUUGAUUUCCUCUUUGUUCACAGUGGCUAAGUUCUGCACCUGAGGGGAGCAUGACCCAGACGAGCGC
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Structure
        10          20        30        40        50        60  
CUCUCUCUCUCUCUCUC--|  AGC    CA             AUUG       GUGAUUGA 
                   UCC   CAGG  CAGAGCUUAGCUG    GUGAACA        \
                   AGG   GUCC  GUCUUGAAUCGGU    CACUUGU        U
CGCGAGCAGACCCAGUACG^  GGA    AC             GA--       UUCUCCUU 
 120       110       100        90        80          70
Deep sequencing
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3' NTU No
MotifsUGUG in loop
Tissue expression
-   +
Sp Sp Sp To
Star sequence

Tni-Mir-27-P2b_5p*
mirBase accessionNone
Sequence 
0- AGAGCUUAGCUGAUUGGUGAACA -23
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Mature sequence

Tni-Mir-27-P2b_3p
mirBase accessionMIMAT0002952
Sequence 
42- UUCACAGUGGCUAAGUUCUGC -63
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