MirGeneDB 3.0
MirGeneDB ID

Esc-Mir-2-o47a

Family name MIR-2 (all species)
Species Hawaiian bobtail squid (Euprymna scolopes)
MiRBase ID
Paralogues Esc-Mir-2-o45-v1  Esc-Mir-2-o47a-v1  Esc-Mir-2-o47b  Esc-Mir-2-o47c  Esc-Mir-2-o47d  Esc-Mir-2-o48-v1  Esc-Mir-2-o49  Esc-Mir-2-P12 
Orthologues Gsp-Mir-2  Npo-Mir-2-o47  Obi-Mir-2-o47a  Ovu-Mir-2-o47a  Ple-Mir-2 
Node of Origin (locus) Coleoidea
Node of Origin (family) Protostomia
Genome context
(esc-GCA_024364805.1_ASM2436480v1)		 CM044488.1: 27564797-27564854 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o47a-v2)		 Mir-2-o49 CM044488.1: 27562752-27562815 [-] Ensembl
Mir-2-o48-v1 CM044488.1: 27562855-27562918 [-] Ensembl
Mir-2-o48-v2 CM044488.1: 27562855-27562918 [-] Ensembl
Mir-2-o47d CM044488.1: 27563803-27563860 [-] Ensembl
Mir-2-o47c CM044488.1: 27564000-27564059 [-] Ensembl
Mir-2-o47b CM044488.1: 27564469-27564528 [-] Ensembl
Mir-2-o47a-v1 CM044488.1: 27564797-27564854 [-] Ensembl
Mir-2-o47a-v2 CM044488.1: 27564797-27564854 [-] Ensembl
Mir-2-P12 CM044488.1: 27565399-27565460 [-] Ensembl
Mir-2-o45-v1 CM044488.1: 27565591-27565650 [-] Ensembl
Mir-2-o45-v2 CM044488.1: 27565591-27565650 [-] Ensembl
Mir-71 CM044488.1: 27565864-27565922 [-] Ensembl
Variant

Esc-Mir-2-o47a-v2
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
UUUUACCCAAGAGAGAAGGCACUGUUGCAACUCAUCAAAUAGCCGUGAUAUGCUGACAAUGACUUAUCACAGCUAGCUUUGAUGAGCUGGGACUCUGCUCUUUGCUGCUUCUGUUUUA
Get precursor sequence
Structure
        10          20        30          40        50       
UUUUACCCAAGAGAGAA--    CU   G  A         --|   C      UGCUGA 
                   GGCA  GUU CA CUCAUCAAA  UAGC GUGAUA      C
                   UCGU  CAG GU GAGUAGUUU  AUCG CACUAU      A
AUUUUGUCUUCGUCGUUUC    CU   G  C         CG^   A      UCAGUA 
      110       100        90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
Li
Star sequence

Esc-Mir-2-o47a-v2_5p*
mirBase accessionNone
Sequence 
0- CUCAUCAAAUAGCCGUGAUAUG -22
Get sequence
Mature sequence

Esc-Mir-2-o47a-v2_3p
mirBase accessionNone
Sequence 
34- UAUCACAGCUAGCUUUGAUGAGCU -58
Get sequence
Variant

Esc-Mir-2-o47a-v1
Seed CACAGCU
Precursor
(pre-Mir +30nt flank)		 
UUUUACCCAAGAGAGAAGGCACUGUUGCAACUCAUCAAAUAGCCGUGAUAUGCUGACAAUGACUUAUCACAGCUAGCUUUGAUGAGCUGGGACUCUGCUCUUUGCUGCUUCUGUUUUA
Get precursor sequence
Structure
        10          20        30          40        50       
UUUUACCCAAGAGAGAA--    CU   G  A         --|   C      UGCUGA 
                   GGCA  GUU CA CUCAUCAAA  UAGC GUGAUA      C
                   UCGU  CAG GU GAGUAGUUU  AUCG CACUAU      A
AUUUUGUCUUCGUCGUUUC    CU   G  C         CG^   A      UCAGUA 
      110       100        90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
Li
Star sequence

Esc-Mir-2-o47a-v1_5p*
mirBase accessionNone
Sequence 
0- CUCAUCAAAUAGCCGUGAUA -20
Get sequence
Mature sequence

Esc-Mir-2-o47a-v1_3p
mirBase accessionNone
Sequence 
36- UCACAGCUAGCUUUGAUGAGCU -58
Get sequence