MirGeneDB 3.0

MirGeneDB ID

Esc-Mir-2-o45

Family name MIR-2 (all species)
Species Hawaiian bobtail squid (Euprymna scolopes)
MiRBase ID
Paralogues Esc-Mir-2-o45-v1  Esc-Mir-2-o47a-v1  Esc-Mir-2-o47b  Esc-Mir-2-o47c  Esc-Mir-2-o47d  Esc-Mir-2-o48-v1  Esc-Mir-2-o49  Esc-Mir-2-P12 
Orthologues Gsp-Mir-2  Npo-Mir-2-o45  Obi-Mir-2-o45-v1  Ovu-Mir-2-o45-v1  Ple-Mir-2 
Node of Origin (locus) Cephalopoda
Node of Origin (family) Protostomia
Genome context
(esc-GCA_024364805.1_ASM2436480v1)
CM044488.1: 27565591-27565650 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o45-v2)
Mir-2-o49 CM044488.1: 27562752-27562815 [-] Ensembl
Mir-2-o48-v1 CM044488.1: 27562855-27562918 [-] Ensembl
Mir-2-o48-v2 CM044488.1: 27562855-27562918 [-] Ensembl
Mir-2-o47d CM044488.1: 27563803-27563860 [-] Ensembl
Mir-2-o47c CM044488.1: 27564000-27564059 [-] Ensembl
Mir-2-o47b CM044488.1: 27564469-27564528 [-] Ensembl
Mir-2-o47a-v1 CM044488.1: 27564797-27564854 [-] Ensembl
Mir-2-o47a-v2 CM044488.1: 27564797-27564854 [-] Ensembl
Mir-2-P12 CM044488.1: 27565399-27565460 [-] Ensembl
Mir-2-o45-v1 CM044488.1: 27565591-27565650 [-] Ensembl
Mir-2-o45-v2 CM044488.1: 27565591-27565650 [-] Ensembl
Mir-71 CM044488.1: 27565864-27565922 [-] Ensembl
Variant

Esc-Mir-2-o45-v2

Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
AAAAACCUGUAUGAGGAGCAGGUUGCGAAGGCAUCAAUGCUGGAUGUCAUAGUAAUCUCCUUGGCCUAUCACAGCCAGCUUUGAUGAGCCUCGUCUCCUGCCAAGCAUCACUGACUUUUU
Get precursor sequence
Structure
        10          20        30          40        50         
AAAAACCUGUAUGAGGA--     UU   A    --|    U     A   C    UAAUCU 
                   GCAGG  GCG AGGC  AUCAA GCUGG UGU AUAG      \
                   CGUCC  UGC UCCG  UAGUU CGACC ACA UAUC      C
UUUUUCAGUCACUACGAAC     UC   -    AG^    U     G   C    CGGUUC 
.       110       100         90        80        70        60
Deep sequencing
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Star sequence

Esc-Mir-2-o45-v2_5p* (predicted)

mirBase accessionNone
Sequence
0- GCAUCAAUGCUGGAUGUCAUAGU -23
Get sequence
Mature sequence

Esc-Mir-2-o45-v2_3p

mirBase accessionNone
Sequence
36- UAUCACAGCCAGCUUUGAUGAGCC -60
Get sequence
Variant

Esc-Mir-2-o45-v1

Seed CACAGCC
Precursor
(pre-Mir +30nt flank)
AAAAACCUGUAUGAGGAGCAGGUUGCGAAGGCAUCAAUGCUGGAUGUCAUAGUAAUCUCCUUGGCCUAUCACAGCCAGCUUUGAUGAGCCUCGUCUCCUGCCAAGCAUCACUGACUUUUU
Get precursor sequence
Structure
        10          20        30          40        50         
AAAAACCUGUAUGAGGA--     UU   A    --|    U     A   C   GUAAUCU 
                   GCAGG  GCG AGGC  AUCAA GCUGG UGU AUA       \
                   CGUCC  UGC UCCG  UAGUU CGACC ACA UAU       C
UUUUUCAGUCACUACGAAC     UC   -    AG^    U     G   C   CCGGUUC 
.       110       100         90        80        70        60
Deep sequencing
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Star sequence

Esc-Mir-2-o45-v1_5p* (predicted)

mirBase accessionNone
Sequence
0- GCAUCAAUGCUGGAUGUCAUA -21
Get sequence
Mature sequence

Esc-Mir-2-o45-v1_3p

mirBase accessionNone
Sequence
38- UCACAGCCAGCUUUGAUGAGCC -60
Get sequence