MirGeneDB 3.0
MirGeneDB ID

Obi-Mir-2-o45

Family name MIR-2 (all species)
Species California two-spot octopus (Octopus bimaculoides)
MiRBase ID
Paralogues Obi-Mir-2-o46-v1  Obi-Mir-2-o47a  Obi-Mir-2-o47b  Obi-Mir-2-o47c  Obi-Mir-2-o47d  Obi-Mir-2-o48-v1  Obi-Mir-2-o49  Obi-Mir-2-P12 
Orthologues Esc-Mir-2-o45-v1  Gsp-Mir-2  Npo-Mir-2-o45  Ovu-Mir-2-o45-v1  Ple-Mir-2 
Node of Origin (locus) Cephalopoda
Node of Origin (family) Protostomia
Genome context
(PRJNA270931_OBI)		 KQ419829: 51363-51423 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o45-v1)		 Mir-2-o49 KQ419829: 49553-49612 [-] Ensembl
Mir-2-o48-v2 KQ419829: 49651-49710 [-] Ensembl
Mir-2-o48-v1 KQ419829: 49651-49710 [-] Ensembl
Mir-2-o47d KQ419829: 50094-50153 [-] Ensembl
Mir-2-o47c KQ419829: 50292-50351 [-] Ensembl
Mir-2-o47b KQ419829: 50497-50556 [-] Ensembl
Mir-2-o47a KQ419829: 50806-50865 [-] Ensembl
Mir-2-o46-v2 KQ419829: 51011-51070 [-] Ensembl
Mir-2-o46-v1 KQ419829: 51011-51070 [-] Ensembl
Mir-2-P12 KQ419829: 51162-51227 [-] Ensembl
Mir-2-o45-v1 KQ419829: 51363-51423 [-] Ensembl
Mir-2-o45-v2 KQ419829: 51363-51423 [-] Ensembl
Mir-71 KQ419829: 51632-51690 [-] Ensembl
Variant

Obi-Mir-2-o45-v1
Seed CACAGCC
Precursor
(pre-Mir +30nt flank)		 
CAGAGCUUGAUGUAGAAACAGGUUACAAAGGCAUCAAUGCUGGAUGUCAUAGUAAAUCUAUGGGGCCUAUCACAGCCAGCUUUGAUGAGCCUUGUCUCCUGCCAGUCAUCACUGCUAGUUU
Get precursor sequence
Structure
        10          20        30          40        50         
CAGAGCUUGAUGUAGAAA--    UU  A     --|    U     A   C   GUAAAUC 
                    CAGG  AC AAGGC  AUCAA GCUGG UGU AUA       U
                    GUCC  UG UUCCG  UAGUU CGACC ACA UAU       A
UUUGAUCGUCACUACUGACC    UC  -     AG^    U     G   C   CCGGGGU 
 .       110       100         90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Obi-Mir-2-o45-v1_5p* (predicted)
mirBase accessionNone
Sequence 
0- GCAUCAAUGCUGGAUGUCAUA -21
Get sequence
Mature sequence

Obi-Mir-2-o45-v1_3p
mirBase accessionNone
Sequence 
39- UCACAGCCAGCUUUGAUGAGCC -61
Get sequence
Variant

Obi-Mir-2-o45-v2
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
CAGAGCUUGAUGUAGAAACAGGUUACAAAGGCAUCAAUGCUGGAUGUCAUAGUAAAUCUAUGGGGCCUAUCACAGCCAGCUUUGAUGAGCCUUGUCUCCUGCCAGUCAUCACUGCUAGUUU
Get precursor sequence
Structure
        10          20        30          40        50         
CAGAGCUUGAUGUAGAAA--    UU  A     --|    U     A   C    UAAAUC 
                    CAGG  AC AAGGC  AUCAA GCUGG UGU AUAG      U
                    GUCC  UG UUCCG  UAGUU CGACC ACA UAUC      A
UUUGAUCGUCACUACUGACC    UC  -     AG^    U     G   C    CGGGGU 
 .       110       100         90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Obi-Mir-2-o45-v2_5p* (predicted)
mirBase accessionNone
Sequence 
0- GCAUCAAUGCUGGAUGUCAUAGU -23
Get sequence
Mature sequence

Obi-Mir-2-o45-v2_3p
mirBase accessionNone
Sequence 
37- UAUCACAGCCAGCUUUGAUGAGCC -61
Get sequence