MirGeneDB 3.0
MirGeneDB ID

Obi-Mir-2-o48

Family name MIR-2 (all species)
Species California two-spot octopus (Octopus bimaculoides)
MiRBase ID
Paralogues Obi-Mir-2-o45-v1  Obi-Mir-2-o46-v1  Obi-Mir-2-o47a  Obi-Mir-2-o47b  Obi-Mir-2-o47c  Obi-Mir-2-o47d  Obi-Mir-2-o48-v1  Obi-Mir-2-o49  Obi-Mir-2-P12 
Orthologues Esc-Mir-2-o48-v1  Gsp-Mir-2  Npo-Mir-2-o48a  Npo-Mir-2-o48b  Ovu-Mir-2-o48-v1  Ple-Mir-2 
Node of Origin (locus) Cephalopoda
Node of Origin (family) Protostomia
Genome context
(PRJNA270931_OBI)		 KQ419829: 49651-49710 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o48-v2)		 Mir-2-o49 KQ419829: 49553-49612 [-] Ensembl
Mir-2-o48-v2 KQ419829: 49651-49710 [-] Ensembl
Mir-2-o48-v1 KQ419829: 49651-49710 [-] Ensembl
Mir-2-o47d KQ419829: 50094-50153 [-] Ensembl
Mir-2-o47c KQ419829: 50292-50351 [-] Ensembl
Mir-2-o47b KQ419829: 50497-50556 [-] Ensembl
Mir-2-o47a KQ419829: 50806-50865 [-] Ensembl
Mir-2-o46-v2 KQ419829: 51011-51070 [-] Ensembl
Mir-2-o46-v1 KQ419829: 51011-51070 [-] Ensembl
Mir-2-P12 KQ419829: 51162-51227 [-] Ensembl
Mir-2-o45-v1 KQ419829: 51363-51423 [-] Ensembl
Mir-2-o45-v2 KQ419829: 51363-51423 [-] Ensembl
Mir-71 KQ419829: 51632-51690 [-] Ensembl
Variant

Obi-Mir-2-o48-v2
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
GUUGUCGGGGGCUCUGCUCUCUGCUUUAAGUCACCAAAGUGGUGGUGAUGUGUUCUCACUAAACCAUAUCACAGCCAGCUUUGAUGAGCUCAAACAUGAGACCAUACAGCGAUGGCUGGG
Get precursor sequence
Structure
        10          20         30          40        50        
GUUGUCGGGGGCUCUGC--    -  C   A  -   C     -|   G        UUCUC 
                   UCUC UG UUU AG UCA CAAAG UGGU GUGAUGUG     A
                   AGAG AC AAA UC AGU GUUUC ACCG CACUAUAC     C
GGGUCGGUAGCGACAUACC    U  -   C  G   A     G^   A        CAAAU 
.       110       100         90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Obi-Mir-2-o48-v2_5p*
mirBase accessionNone
Sequence 
0- UCACCAAAGUGGUGGUGAUGUG -22
Get sequence
Mature sequence

Obi-Mir-2-o48-v2_3p
mirBase accessionNone
Sequence 
36- UAUCACAGCCAGCUUUGAUGAGCU -60
Get sequence
Variant

Obi-Mir-2-o48-v1
Seed CACAGCC
Precursor
(pre-Mir +30nt flank)		 
GUUGUCGGGGGCUCUGCUCUCUGCUUUAAGUCACCAAAGUGGUGGUGAUGUGUUCUCACUAAACCAUAUCACAGCCAGCUUUGAUGAGCUCAAACAUGAGACCAUACAGCGAUGGCUGGG
Get precursor sequence
Structure
        10          20         30          40        50        
GUUGUCGGGGGCUCUGC--    -  C   A  -   C     -|   G      UGUUCUC 
                   UCUC UG UUU AG UCA CAAAG UGGU GUGAUG       A
                   AGAG AC AAA UC AGU GUUUC ACCG CACUAU       C
GGGUCGGUAGCGACAUACC    U  -   C  G   A     G^   A      ACCAAAU 
.       110       100         90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17), UGUG in loop
Tissue expression
- +
To
Star sequence

Obi-Mir-2-o48-v1_5p*
mirBase accessionNone
Sequence 
0- UCACCAAAGUGGUGGUGAUG -20
Get sequence
Mature sequence

Obi-Mir-2-o48-v1_3p
mirBase accessionNone
Sequence 
38- UCACAGCCAGCUUUGAUGAGCU -60
Get sequence