MirGeneDB 3.0
MirGeneDB ID

Obi-Mir-2-o46

Family name MIR-2 (all species)
Species California two-spot octopus (Octopus bimaculoides)
MiRBase ID
Paralogues Obi-Mir-2-o45-v1  Obi-Mir-2-o47a  Obi-Mir-2-o47b  Obi-Mir-2-o47c  Obi-Mir-2-o47d  Obi-Mir-2-o48-v1  Obi-Mir-2-o49  Obi-Mir-2-P12 
Orthologues Gsp-Mir-2  Ovu-Mir-2-o46-v1  Ple-Mir-2 
Node of Origin (locus) Octopus
Node of Origin (family) Protostomia
Genome context
(PRJNA270931_OBI)		 KQ419829: 51011-51070 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o46-v1)		 Mir-2-o49 KQ419829: 49553-49612 [-] Ensembl
Mir-2-o48-v2 KQ419829: 49651-49710 [-] Ensembl
Mir-2-o48-v1 KQ419829: 49651-49710 [-] Ensembl
Mir-2-o47d KQ419829: 50094-50153 [-] Ensembl
Mir-2-o47c KQ419829: 50292-50351 [-] Ensembl
Mir-2-o47b KQ419829: 50497-50556 [-] Ensembl
Mir-2-o47a KQ419829: 50806-50865 [-] Ensembl
Mir-2-o46-v2 KQ419829: 51011-51070 [-] Ensembl
Mir-2-o46-v1 KQ419829: 51011-51070 [-] Ensembl
Mir-2-P12 KQ419829: 51162-51227 [-] Ensembl
Mir-2-o45-v1 KQ419829: 51363-51423 [-] Ensembl
Mir-2-o45-v2 KQ419829: 51363-51423 [-] Ensembl
Mir-71 KQ419829: 51632-51690 [-] Ensembl
Variant

Obi-Mir-2-o46-v1
Seed CACAGCC
Precursor
(pre-Mir +30nt flank)		 
AUUGCCUAUUCUCUCCUAACCUUGUUACAGCUCAUCAAAGGACUGUGAUAUAUAGAUAUUUGAACGUCUCACAGCCAGCUUUGAUGAGCUGGCUGUAGGUUGCUUCCCUGCUCCGCUAGC
Get precursor sequence
Structure
        10          20        30           40        50        
AUUGCCUAUUCUCUCCU--     UGUUA            ---| A      UAUAUAGAU 
                   AACCU     CAGCUCAUCAAA   GG CUGUGA         A
                   UUGGA     GUCGAGUAGUUU   CC GACACU         U
CGAUCGCCUCGUCCCUUCG     UGUCG            CGA^ -      CUGCAAGUU 
.       110       100        90        80         70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Obi-Mir-2-o46-v1_5p*
mirBase accessionNone
Sequence 
0- CUCAUCAAAGGACUGUGAUA -20
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Mature sequence

Obi-Mir-2-o46-v1_3p
mirBase accessionNone
Sequence 
38- UCACAGCCAGCUUUGAUGAGCU -60
Get sequence
Variant

Obi-Mir-2-o46-v2
Seed CUCACAG
Precursor
(pre-Mir +30nt flank)		 
AUUGCCUAUUCUCUCCUAACCUUGUUACAGCUCAUCAAAGGACUGUGAUAUAUAGAUAUUUGAACGUCUCACAGCCAGCUUUGAUGAGCUGGCUGUAGGUUGCUUCCCUGCUCCGCUAGC
Get precursor sequence
Structure
        10          20        30           40        50        
AUUGCCUAUUCUCUCCU--     UGUUA            ---| A      UAUAUAGAU 
                   AACCU     CAGCUCAUCAAA   GG CUGUGA         A
                   UUGGA     GUCGAGUAGUUU   CC GACACU         U
CGAUCGCCUCGUCCCUUCG     UGUCG            CGA^ -      CUGCAAGUU 
.       110       100        90        80         70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Obi-Mir-2-o46-v2_5p*
mirBase accessionNone
Sequence 
0- CUCAUCAAAGGACUGUGAUAUA -22
Get sequence
Mature sequence

Obi-Mir-2-o46-v2_3p
mirBase accessionNone
Sequence 
36- UCUCACAGCCAGCUUUGAUGAGCU -60
Get sequence