MirGeneDB 3.0
MirGeneDB ID

Llo-Mir-2-o114

Family name MIR-2 (all species)
Species Bootlace worm (Lineus longissimus)
MiRBase ID
Paralogues Llo-Mir-2-o114-v1  Llo-Mir-2-o115  Llo-Mir-2-o116  Llo-Mir-2-o117  Llo-Mir-2-o118  Llo-Mir-2-o119  Llo-Mir-2-o120  Llo-Mir-2-o121  Llo-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) Lineidae
Node of Origin (family) Protostomia
Genome context
(GCA_910592395.2_tnLinLong1)		 OU343002.1: 11598647-11598703 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o114-v2)		 Mir-2-o117 OU343002.1: 11593640-11593697 [-] Ensembl
Mir-2-o116 OU343002.1: 11594484-11594542 [-] Ensembl
Mir-2-o115 OU343002.1: 11595818-11595877 [-] Ensembl
Mir-2-P12 OU343002.1: 11596059-11596116 [-] Ensembl
Mir-2-o114-v1 OU343002.1: 11598646-11598704 [-] Ensembl
Mir-2-o114-v2 OU343002.1: 11598647-11598703 [-] Ensembl
Mir-71 OU343002.1: 11598853-11598911 [-] Ensembl
Variant

Llo-Mir-2-o114-v2
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
CCUUGUCUGUCGAAGUAGCUUGUCCCUUGCUGUCAAUGCUGGACGUAGUAGACAUUCUAAUACCUAUCACAGCCAGCUUUGAUGAGCCAGGUGCAAGUCUGUUGCUGAAAAACAAGU
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Structure
        10          20        30         40        50       
CCUUGUCUGUCGAAGUA--     UC   U   -|    U     AC  A    ACAUU 
                   GCUUG  CCU GCU GUCAA GCUGG  GU GUAG     C
                   UGAAC  GGA CGA UAGUU CGACC  CA UAUC     U
UGAACAAAAAGUCGUUGUC     GU   C   G^    U     GA  C    CAUAA 
     110       100        90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsNo
Tissue expression
- +
No No No No No No
Star sequence

Llo-Mir-2-o114-v2_5p* (predicted)
mirBase accessionNone
Sequence 
0- UGUCAAUGCUGGACGUAGUAGA -22
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Mature sequence

Llo-Mir-2-o114-v2_3p
mirBase accessionNone
Sequence 
34- UAUCACAGCCAGCUUUGAUGAGC -57
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Variant

Llo-Mir-2-o114-v1
Seed CACAGCC
Precursor
(pre-Mir +30nt flank)		 
UCCUUGUCUGUCGAAGUAGCUUGUCCCUUGCUGUCAAUGCUGGACGUAGUAGACAUUCUAAUACCUAUCACAGCCAGCUUUGAUGAGCCAGGUGCAAGUCUGUUGCUGAAAAACAAGUC
Get precursor sequence
Structure
        10          20        30         40        50        
UCCUUGUCUGUCGAAGUA--     UC   U   -|    U     AC  A   GACAUU 
                    GCUUG  CCU GCU GUCAA GCUGG  GU GUA      C
                    UGAAC  GGA CGA UAGUU CGACC  CA UAU      U
CUGAACAAAAAGUCGUUGUC     GU   C   G^    U     GA  C   CCAUAA 
       110       100        90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsNo
Tissue expression
- +
No No No No No No
Star sequence

Llo-Mir-2-o114-v1_5p* (predicted)
mirBase accessionNone
Sequence 
0- CUGUCAAUGCUGGACGUAGUA -21
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Mature sequence

Llo-Mir-2-o114-v1_3p
mirBase accessionNone
Sequence 
37- UCACAGCCAGCUUUGAUGAGCC -59
Get sequence