MirGeneDB 3.0

MirGeneDB ID

Mgi-Mir-2-o61

Family name MIR-2 (all species)
Species Pacific oyster (Magallana gigas)
MiRBase ID
Paralogues Mgi-Mir-2-o56  Mgi-Mir-2-o57  Mgi-Mir-2-o58a  Mgi-Mir-2-o58b  Mgi-Mir-2-o59  Mgi-Mir-2-o60  Mgi-Mir-2-o62  Mgi-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) C. gigas
Node of Origin (family) Protostomia
Genome context
(CGI_GCA_000297895.1)
scaffold602: 446534-446592 [+] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o61)
Mir-71 scaffold602: 444840-444896 [+] Ensembl
Mir-2-o56 scaffold602: 445001-445057 [+] Ensembl
Mir-2-P12 scaffold602: 445140-445196 [+] Ensembl
Mir-2-o57 scaffold602: 445414-445474 [+] Ensembl
Mir-2-o58b scaffold602: 445724-445782 [+] Ensembl
Mir-2-o59 scaffold602: 445843-445901 [+] Ensembl
Mir-2-o60 scaffold602: 446019-446076 [+] Ensembl
Mir-2-o61 scaffold602: 446534-446592 [+] Ensembl
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
UAUUGUCAAUGAGUACUGAUUGCAUGGCUAGCAUUGAAGUGGCAGUGAUGUGCUGAUGUACUGUCAUAUCACAGCCAGCUUUGAUGACAGGCAAGUAAUCUUCACAGCUAAAUAGUCAA
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Structure
        10          20        30         40        50        
UAUUGUCAAUGAGUACU--      A  G  AG   UG   -|   A        CUGAU 
                   GAUUGC UG CU  CAU  AAG UGGC GUGAUGUG     G
                   CUAAUG AC GA  GUA  UUC ACCG CACUAUAC     U
AACUGAUAAAUCGACACUU      A  G  CA   GU   G^   A        UGUCA 
       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUG at 5p(-14), CNNC at 3p(+17)
Tissue expression
- +
A- D- D- F- L- Ea Eg Fe Gi Ha He Ju La Ma Ma Ma Pe Re Sp Um
Star sequence

Mgi-Mir-2-o61_5p*

mirBase accessionNone
Sequence
0- GCAUUGAAGUGGCAGUGAUGUG -22
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Mature sequence

Mgi-Mir-2-o61_3p

mirBase accessionNone
Sequence
36- UAUCACAGCCAGCUUUGAUGACA -59
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