MirGeneDB 3.0

MirGeneDB ID

Mgi-Mir-2-o60

Family name MIR-2 (all species)
Species Pacific oyster (Magallana gigas)
MiRBase ID
Paralogues Mgi-Mir-2-o56  Mgi-Mir-2-o57  Mgi-Mir-2-o58a  Mgi-Mir-2-o58b  Mgi-Mir-2-o59  Mgi-Mir-2-o61  Mgi-Mir-2-o62  Mgi-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) C. gigas
Node of Origin (family) Protostomia
Genome context
(CGI_GCA_000297895.1)
scaffold602: 446019-446076 [+] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o60)
Mir-71 scaffold602: 444840-444896 [+] Ensembl
Mir-2-o56 scaffold602: 445001-445057 [+] Ensembl
Mir-2-P12 scaffold602: 445140-445196 [+] Ensembl
Mir-2-o57 scaffold602: 445414-445474 [+] Ensembl
Mir-2-o58b scaffold602: 445724-445782 [+] Ensembl
Mir-2-o59 scaffold602: 445843-445901 [+] Ensembl
Mir-2-o60 scaffold602: 446019-446076 [+] Ensembl
Mir-2-o61 scaffold602: 446534-446592 [+] Ensembl
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
AAGUCGUGGUGAUAAAGAGACAAAUGGAAGUUGUCAAAGUGGUGGUGAUGUGUUGUCAUGUUCGUAUCACAGCCAGCUUUGAUGAGCUUGAAUUUUGUCGCUGUCGGCAGGGGGAGGG
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Structure
        10         20         30          40        50       
AAGUCGUGGUGAUAAAGA-      UGG-     -       -|   G        UUGU 
                   GACAAA    AAGUU GUCAAAG UGGU GUGAUGUG    C
                   CUGUUU    UUCGA UAGUUUC ACCG CACUAUGC    A
GGGAGGGGGACGGCUGUCG      UAAG     G       G^   A        UUGU 
      110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
A- D- D- F- L- Ea Eg Fe Gi Ha He Ju La Ma Ma Ma Pe Re Sp Um
Star sequence

Mgi-Mir-2-o60_5p*

mirBase accessionNone
Sequence
0- UUGUCAAAGUGGUGGUGAUGUG -22
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Mature sequence

Mgi-Mir-2-o60_3p

mirBase accessionNone
Sequence
34- UAUCACAGCCAGCUUUGAUGAGCU -58
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