MirGeneDB 3.0
MirGeneDB ID

Pdu-Mir-2-o27

Family name MIR-2 (all species)
Species Dumerils clam worm (Platynereis dumerilii)
MiRBase ID
Paralogues Pdu-Mir-2-o25-v1  Pdu-Mir-2-o26  Pdu-Mir-2-o28  Pdu-Mir-2-o29  Pdu-Mir-2-o30  Pdu-Mir-2-o31  Pdu-Mir-2-o32  Pdu-Mir-2-o33  Pdu-Mir-2-o34  Pdu-Mir-2-o35  Pdu-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) P. dumerilii
Node of Origin (family) Protostomia
Genome context
(GCA_026936325.1_EMBL_pdum_1.0)		 JAPTHN010000003.1: 67087901-67087958 [+] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o27)		 Mir-71 JAPTHN010000003.1: 67085298-67085356 [+] Ensembl
Mir-2-o25-v1 JAPTHN010000003.1: 67086277-67086336 [+] Ensembl
Mir-2-o25-v2 JAPTHN010000003.1: 67086277-67086337 [+] Ensembl
Mir-2-P12 JAPTHN010000003.1: 67087096-67087160 [+] Ensembl
Mir-2-o26 JAPTHN010000003.1: 67087490-67087548 [+] Ensembl
Mir-2-o27 JAPTHN010000003.1: 67087901-67087958 [+] Ensembl
Mir-2-o28 JAPTHN010000003.1: 67088098-67088157 [+] Ensembl
Mir-2-o29 JAPTHN010000003.1: 67088403-67088463 [+] Ensembl
Mir-2-o30 JAPTHN010000003.1: 67088840-67088898 [+] Ensembl
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
UAAAUGAUGCACACAGAACGGUACUCAUGACCAUCUAAGUGGCUGUGAUGUGGUGUUGCGAUGCAUAUCACAGCCAGCUUUGAUGAGCAGGGGUAUUGGCCAAUCAGCUGCUGAUUUC
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Structure
        10          20        30         40        50        
UAAAUGAUGCACACAGAA--        A  AC    U   -|            GUGUU 
                    CGGUACUC UG  CAUC AAG UGGCUGUGAUGUG     \
                    GUUAUGGG AC  GUAG UUC ACCGACACUAUAC     G
CUUUAGUCGUCGACUAACCG        G  GA    U   G^            GUAGC 
      110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17), UGUG in loop
Tissue expression
- +
24 24 24 24 24 36 36 36 36 36 48 48 48 48 48 6d 6d 6d 6d 72 72 72 72 72
Star sequence

Pdu-Mir-2-o27_5p*
mirBase accessionNone
Sequence 
0- CCAUCUAAGUGGCUGUGAUGUG -22
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Mature sequence

Pdu-Mir-2-o27_3p
mirBase accessionNone
Sequence 
35- UAUCACAGCCAGCUUUGAUGAGC -58
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