MirGeneDB 3.0
MirGeneDB ID

Pdu-Mir-2-o25

Family name MIR-2 (all species)
Species Dumerils clam worm (Platynereis dumerilii)
MiRBase ID
Paralogues Pdu-Mir-2-o25-v1  Pdu-Mir-2-o26  Pdu-Mir-2-o27  Pdu-Mir-2-o28  Pdu-Mir-2-o29  Pdu-Mir-2-o30  Pdu-Mir-2-o31  Pdu-Mir-2-o32  Pdu-Mir-2-o33  Pdu-Mir-2-o34  Pdu-Mir-2-o35  Pdu-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) P. dumerilii
Node of Origin (family) Protostomia
Genome context
(GCA_026936325.1_EMBL_pdum_1.0)		 JAPTHN010000003.1: 67086277-67086337 [+] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o25-v2)		 Mir-71 JAPTHN010000003.1: 67085298-67085356 [+] Ensembl
Mir-2-o25-v1 JAPTHN010000003.1: 67086277-67086336 [+] Ensembl
Mir-2-o25-v2 JAPTHN010000003.1: 67086277-67086337 [+] Ensembl
Mir-2-P12 JAPTHN010000003.1: 67087096-67087160 [+] Ensembl
Mir-2-o26 JAPTHN010000003.1: 67087490-67087548 [+] Ensembl
Mir-2-o27 JAPTHN010000003.1: 67087901-67087958 [+] Ensembl
Mir-2-o28 JAPTHN010000003.1: 67088098-67088157 [+] Ensembl
Mir-2-o29 JAPTHN010000003.1: 67088403-67088463 [+] Ensembl
Mir-2-o30 JAPTHN010000003.1: 67088840-67088898 [+] Ensembl
Variant

Pdu-Mir-2-o25-v2
Seed CACAGCC
Precursor
(pre-Mir +30nt flank)		 
UAUGGGUGAUGGAGGUGGCGGUGGUCUUGGCAUCAAUCCUGGCAUGUUAUAGUAUCCACAGUCGAGCUAUCACAGCCAGCUUUGAUGAGCCAGGCGCAUUCGCGGGGCAACUCUUUUUAAA
Get precursor sequence
Structure
        10         20          30          40        50         
UAUGGGUGAUGGAGGUG-   -   - U      --|    UC     A   U   GUAUCCA 
                  GCG GUG G CUUGGC  AUCAA  CUGGC UGU AUA       C
                  CGC UAC C GGACCG  UAGUU  GACCG ACA UAU       A
AAAUUUUUCUCAACGGGG   U   G -      AG^    UC     -   C   CGAGCUG 
 .       110       100         90        80         70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
24 24 24 24 24 36 36 36 36 36 48 48 48 48 48 6d 6d 6d 6d 72 72 72 72 72
Star sequence

Pdu-Mir-2-o25-v2_5p*
mirBase accessionNone
Sequence 
0- CAUCAAUCCUGGCAUGUUAUA -21
Get sequence
Mature sequence

Pdu-Mir-2-o25-v2_3p
mirBase accessionNone
Sequence 
39- UCACAGCCAGCUUUGAUGAGCC -61
Get sequence
Variant

Pdu-Mir-2-o25-v1
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
UAUGGGUGAUGGAGGUGGCGGUGGUCUUGGCAUCAAUCCUGGCAUGUUAUAGUAUCCACAGUCGAGCUAUCACAGCCAGCUUUGAUGAGCCAGGCGCAUUCGCGGGGCAACUCUUUUUAA
Get precursor sequence
Structure
        10              20        30          40        50         
UAUGGGUGAUGGAGGUG------|  GUGGU      --     UC     A   U     AUCCA 
                       GCG     CUUGGC  AUCAA  CUGGC UGU AUAGU     C
                       CGC     GGACCG  UAGUU  GACCG ACA UAUCG     A
AAUUUUUCUCAACGGGGCGCUUA^  -----      AG     UC     -   C     AGCUG 
.       110       100             90        80         70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
24 24 24 24 24 36 36 36 36 36 48 48 48 48 48 6d 6d 6d 6d 72 72 72 72 72
Star sequence

Pdu-Mir-2-o25-v1_5p*
mirBase accessionNone
Sequence 
0- CAUCAAUCCUGGCAUGUUAUAGU -23
Get sequence
Mature sequence

Pdu-Mir-2-o25-v1_3p
mirBase accessionNone
Sequence 
37- UAUCACAGCCAGCUUUGAUGAGC -60
Get sequence