MirGeneDB 3.0
MirGeneDB ID

Eca-Mir-421-P1

Family name MIR-421 (all species)
Species Horse (Equus caballus)
MiRBase ID eca-mir-545
Paralogues Eca-Mir-421-P1-v1  Eca-Mir-421-P2 
Orthologues Bta-Mir-421-P1-v1  Cfa-Mir-421-P1-v1  Cpo-Mir-421-P1  Dno-Mir-421-P1-v1  Hsa-Mir-421-P1-v1  Mml-Mir-421-P1-v1  Mmr-Mir-421-P1a  Mmr-Mir-421-P1b  Ocu-Mir-421-P1-v1  Pab-Mir-421-P1 
Node of Origin (locus) Eutheria
Node of Origin (family) Eutheria
Genome context
(GCA_002863925.1_EquCab3.0)		 CM009179.1: 58620606-58620665 [-] UCSC Ensembl
Clustered miRNAs
(< 50kb from Mir-421-P1-v2)		 Mir-421-P1-v1 CM009179.1: 58620605-58620667 [-] UCSC Ensembl
Mir-421-P1-v2 CM009179.1: 58620606-58620665 [-] UCSC Ensembl
Mir-374-P1 CM009179.1: 58620777-58620828 [-] UCSC Ensembl
Variant

Eca-Mir-421-P1-v2
Seed CAGUAAA
Precursor
(pre-Mir +30nt flank)		 
CCAGAGCCCAGCCUGGCACAUUAGUAGGCCUCAGUAAAUGUUUAUUGGAUGAAUAAAUGAAUGACUCAUCAACAAACAUUUAUUGUGUGCCUGCUAACGUGAUCUCCACAGGCUCUGGAC
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Structure
        10           20        30         40        50         
CCAGAGCCCAGCCUGGC---  A         CU-|           AUUG     AUAAAU 
                    AC UUAGUAGGC   CAGUAAAUGUUU    GAUGA      \
                    UG AAUCGUCCG   GUUAUUUACAAA    CUACU      G
CAGGUCUCGGACACCUCUAG  C         UGU^           CAA-     CAGUAA 
.       110       100        90        80         70        60
Deep sequencing
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3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
Ps Bo Ca Em En En En En En En En Gl He He Ho Im Ki Li Lo Lu Ma Ma Mi Pl Sp Te Tr Ut Ut Yo
Mature sequence

Eca-Mir-421-P1-v2_5p
mirBase accessionNone
Sequence 
0- UCAGUAAAUGUUUAUUGGAUGA -22
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Co-mature sequence

Eca-Mir-421-P1-v2_3p
mirBase accessionMIMAT0013239
Sequence 
37- AUCAACAAACAUUUAUUGUGUGC -60
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Variant

Eca-Mir-421-P1-v1
Seed CAACAAA
Precursor
(pre-Mir +30nt flank)		 
CUCCAGAGCCCAGCCUGGCACAUUAGUAGGCCUCAGUAAAUGUUUAUUGGAUGAAUAAAUGAAUGACUCAUCAACAAACAUUUAUUGUGUGCCUGCUAACGUGAUCUCCACAGGCUCUGGACU
Get precursor sequence
Structure
        10          20        30         40        50        60 
CUCCAGAGCCCAGCCUGG--   A         CU-|           AUUG    AAUAAAU 
                    CAC UUAGUAGGC   CAGUAAAUGUUU    GAUG       \
                    GUG AAUCGUCCG   GUUAUUUACAAA    CUAC       G
UCAGGUCUCGGACACCUCUA   C         UGU^           CAA-    UCAGUAA 
 120       110       100        90        80         70
Deep sequencing
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3' NTU No
MotifsNo
Tissue expression
- +
Ps Bo Ca Em En En En En En En En Gl He He Ho Im Ki Li Lo Lu Ma Ma Mi Pl Sp Te Tr Ut Ut Yo
Star sequence

Eca-Mir-421-P1-v1_5p*
mirBase accessionNone
Sequence 
0- CCUCAGUAAAUGUUUAUUGGAUG -23
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Mature sequence

Eca-Mir-421-P1-v1_3p
mirBase accessionMIMAT0013239
Sequence 
40- UCAACAAACAUUUAUUGUGUGCC -63
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