MirGeneDB 3.0

MirGeneDB ID

Rph-Mir-2-o70

Family name MIR-2 (all species)
Species Japanese carpet shell (Ruditapes philippinarum)
MiRBase ID
Paralogues Rph-Mir-2-o63  Rph-Mir-2-o64  Rph-Mir-2-o65  Rph-Mir-2-o66  Rph-Mir-2-o67  Rph-Mir-2-o68  Rph-Mir-2-o69  Rph-Mir-2-o71  Rph-Mir-2-o72  Rph-Mir-2-o73  Rph-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) R. philippinarum
Node of Origin (family) Protostomia
Genome context
(Ruditapes_GCA_009026015)
QUSP01002410.1: 60291-60350 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o70)
Mir-2-o71 QUSP01002410.1: 60139-60197 [-] Ensembl
Mir-2-o70 QUSP01002410.1: 60291-60350 [-] Ensembl
Mir-2-o69 QUSP01002410.1: 63485-63542 [-] Ensembl
Mir-2-o68 QUSP01002410.1: 64540-64597 [-] Ensembl
Mir-2-o67 QUSP01002410.1: 64738-64796 [-] Ensembl
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
UGGGAGCAGUUGCAGAGAAAAAUGAAACGCCCGUCAAAGUGGCUGUGAACUGGUGUGUAAUAUUUCAGAUCACAGCCUGCUUUGAUGAGCCUUUCUGAAGCACAUCAGGAUUACACUAUU
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Structure
        10        20          30        40         50         
UGGGAGCAGUUGCAGAGAAAAAU--    C  C         -|       A   GUGUGU 
                         GAAA GC CGUCAAAGU GGCUGUGA CUG      \
                         CUUU CG GUAGUUUCG CCGACACU GAC      A
UUAUCACAUUAGGACUACACGAAGU    C  A         U^       A   UUUAUA 
.       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUGUG in loop
Tissue expression
- +
Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru
Star sequence

Rph-Mir-2-o70_5p*

mirBase accessionNone
Sequence
0- CCGUCAAAGUGGCUGUGAACUG -22
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Mature sequence

Rph-Mir-2-o70_3p

mirBase accessionNone
Sequence
37- GAUCACAGCCUGCUUUGAUGAGC -60
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