Mir-2-o47b

MirGeneDB ID

Ovu-Mir-2-o47b

Family name

Species

MiRBase ID

Paralogues

Ovu-Mir-2-o45-v1 Ovu-Mir-2-o46-v1 Ovu-Mir-2-o47a Ovu-Mir-2-o47c Ovu-Mir-2-o47d Ovu-Mir-2-o48-v1 Ovu-Mir-2-o49 Ovu-Mir-2-P12

Orthologues

Esc-Mir-2-o47b Gsp-Mir-2 Npo-Mir-2-o47 Obi-Mir-2-o47b Ple-Mir-2

Node of Origin (locus)

Coleoidea

Node of Origin (family)

Protostomia

Genome context
(GCA_003957725.1_ASM395772v1_OVU)

RXHP01057295.1: 22396-22455 [-] Ensembl

Clustered miRNAs
(< 50kb from Mir-2-o47b)

Mir-2-o49 RXHP01057295.1: 21463-21522 [-] Ensembl
Mir-2-o48-v2 RXHP01057295.1: 21561-21620 [-] Ensembl
Mir-2-o48-v1 RXHP01057295.1: 21561-21620 [-] Ensembl
Mir-2-o47d RXHP01057295.1: 22007-22066 [-] Ensembl
Mir-2-o47c RXHP01057295.1: 22198-22257 [-] Ensembl
Mir-2-o47b RXHP01057295.1: 22396-22455 [-] Ensembl
Mir-2-o47a RXHP01057295.1: 22702-22761 [-] Ensembl
Mir-2-o46-v2 RXHP01057295.1: 22936-22995 [-] Ensembl
Mir-2-o46-v1 RXHP01057295.1: 22936-22995 [-] Ensembl
Mir-2-P12 RXHP01057295.1: 23087-23152 [-] Ensembl
Mir-2-o45-v1 RXHP01057295.1: 23280-23340 [-] Ensembl
Mir-2-o45-v2 RXHP01057295.1: 23280-23340 [-] Ensembl
Mir-71 RXHP01057295.1: 23544-23602 [-] Ensembl

Seed

AUCACAG

Precursor
(pre-Mir +30nt flank)

CUCUGCGUGUAUCUAUUGACCAUGUUGUAUCUCAUCAAAUAACUGGGAUAUGCGGUUAUAAUGACGUAUCACAGCUAGCUUUGAUGAGCUUCAACUUGGUUGUUCUCAGCAACCUGGUUA

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Structure

        10          20        30          40        50        
CUCUGCGUGUAUCUAUU--     U    UAU         --| A   G      CGGUU 
                   GACCA GUUG   CUCAUCAAA  UA CUG GAUAUG     A
                   UUGGU CAAC   GAGUAGUUU  AU GAC CUAUGC     U
AUUGGUCCAACGACUCUUG     U    UUC         CG^ C   A      AGUAA 
.       110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

Ax	Ce	Fr	He	In	Op	Op	Pe	Po	Re	Sk	St	Su	Su	Te	Ve	Vi	Wh

Star sequence

Ovu-Mir-2-o47b_5p*

mirBase accession

None

Sequence

0- CUCAUCAAAUAACUGGGAUAUG -22

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Mature sequence

Ovu-Mir-2-o47b_3p

mirBase accession

None

Sequence

36- UAUCACAGCUAGCUUUGAUGAGCU -60

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