MirGeneDB 3.0

MirGeneDB ID

Ofu-Mir-2-o154

Family name MIR-2 (all species)
Species Tubeworm (Owenia fusiformis)
MiRBase ID
Paralogues Ofu-Mir-2-o153  Ofu-Mir-2-o155  Ofu-Mir-2-o156  Ofu-Mir-2-o157  Ofu-Mir-2-o158  Ofu-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) O. fusiformis
Node of Origin (family) Protostomia
Genome context
(GCA_903813345.2_Owenia)
CAIIXF020000003.1: 15959506-15959565 [+]
Clustered miRNAs
(< 50kb from Mir-2-o154)
Mir-71 CAIIXF020000003.1: 15950516-15950575 [+]
Mir-2-o153 CAIIXF020000003.1: 15958470-15958526 [+]
Mir-2-P12 CAIIXF020000003.1: 15958948-15959009 [+]
Mir-2-o154 CAIIXF020000003.1: 15959506-15959565 [+]
Mir-2-o155 CAIIXF020000003.1: 15960009-15960067 [+]
Mir-2-o156 CAIIXF020000003.1: 15960548-15960608 [+]
Mir-2-o157 CAIIXF020000003.1: 15962486-15962544 [+]
Mir-2-o158 CAIIXF020000003.1: 15964675-15964734 [+]
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
CAAGGUUGAUGAUUUGGGCCAGUUGUAUGGGCAUCAAAUUGGCUGUGAAAUGUUCUUACAUUUUCAUAUCACAGCCAGCUUUGAUGAGCCACACAUGGUUAGUUUGCAUCAUAAAAGGUU
Get precursor sequence
Structure
        10            20        30          40        50        
CAAGGUUGAUGAUUUGG----|   GU   A   G-       -          A   UUCUU 
                     GCCA  UGU UGG  CAUCAAA UUGGCUGUGA AUG     A
                     UGGU  ACA ACC  GUAGUUU GACCGACACU UAC     C
UUGGAAAAUACUACGUUUGAU^   --   C   GA       C          A   UUUUA 
.       110       100          90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To To
Star sequence

Ofu-Mir-2-o154_5p* (predicted)

mirBase accessionNone
Sequence
0- GCAUCAAAUUGGCUGUGAAAUG -22
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Mature sequence

Ofu-Mir-2-o154_3p

mirBase accessionNone
Sequence
36- UAUCACAGCCAGCUUUGAUGAGCC -60
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