MirGeneDB 3.0

MirGeneDB ID

Efe-Mir-2-o21

Family name MIR-2 (all species)
Species Common brandling worm (Eisenia fetida)
MiRBase ID
Paralogues Efe-Mir-2-o20  Efe-Mir-2-o22  Efe-Mir-2-o23  Efe-Mir-2-o24  Efe-Mir-2-P12f  Efe-Mir-2-P12g 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) E. fetida
Node of Origin (family) Protostomia
Genome context
(Efe_combined)
Efet.01.155957: 5781-5841 [+]
Clustered miRNAs
(< 50kb from Mir-2-o21)
Mir-71-P1 Efet.01.155957: 625-687 [+]
Mir-2-P12g Efet.01.155957: 2068-2132 [+]
Mir-2-o21 Efet.01.155957: 5781-5841 [+]
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
GCUAUGUUUUGAAACUUGGUGGUCACAUGGCAGUCAAAGUGGCUGUGAUAUGUAGAACUGACUAAGUGCAUCACAGCCUGCUUUGUCUGGAAUGUGUCUACUGCUGACUCAACUCUAACGC
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Structure
        10          20        30        40         50        60
GCUAUGUUUUGAAACUU--     U     GG   U      -|       UA   AGAACU 
                   GGUGG CACAU  CAG CAAAGU GGCUGUGA  UGU      G
                   UCAUC GUGUA  GUC GUUUCG CCGACACU  ACG      A
CGCAAUCUCAACUCAGUCG     U     AG   U      U^       --   UGAAUC 
 .       110       100        90        80          70
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUG at 5p(-14)
Tissue expression
- +
To
Star sequence

Efe-Mir-2-o21_5p*

mirBase accessionNone
Sequence
0- CAGUCAAAGUGGCUGUGAUAUGU -23
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Mature sequence

Efe-Mir-2-o21_3p

mirBase accessionNone
Sequence
38- CAUCACAGCCUGCUUUGUCUGGA -61
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