MirGeneDB 3.0
MirGeneDB ID

Efe-Mir-2-o23

Family name MIR-2 (all species)
Species Common brandling worm (Eisenia fetida)
MiRBase ID
Paralogues Efe-Mir-2-o20  Efe-Mir-2-o21  Efe-Mir-2-o22  Efe-Mir-2-o24  Efe-Mir-2-P12f  Efe-Mir-2-P12g 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) E. fetida
Node of Origin (family) Protostomia
Genome context
(Efe_combined)		 Efet.01.159362: 1072-1126 [-]
Clustered miRNAs
(< 50kb from Mir-2-o23)		 Mir-2-o24 Efet.01.159362: 648-708 [-]
Mir-2-o23 Efet.01.159362: 1072-1126 [-]
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
NNACCAUGUUAUGGGGAGCAGCUGAAGGCCUGGUCAAAGAUUCUGUGAAAUGCUACUGAAUCAUAUCACAGCCGCUUUGACUGGUUCUUAACUGCAUCACAUCUUCACUUUCUGC
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Structure
        10           20        30        40        50       
NNACCAUGUUAUGGGGA---|   C   AG   U        AUU      A   CUAC 
                    GCAG UGA  GCC GGUCAAAG   CUGUGA AUG    \
                    CGUC AUU  UGG UCAGUUUC   GACACU UAC    U
CGUCUUUCACUUCUACACUA^   A   CU   -        GCC      A   UAAG 
   110       100        90         80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Efe-Mir-2-o23_5p*
mirBase accessionNone
Sequence 
0- UGGUCAAAGAUUCUGUGAAAUG -22
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Mature sequence

Efe-Mir-2-o23_3p
mirBase accessionNone
Sequence 
33- UAUCACAGCCGCUUUGACUGGU -55
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