MirGeneDB 3.0
MirGeneDB ID

Efe-Mir-2-o24

Family name MIR-2 (all species)
Species Common brandling worm (Eisenia fetida)
MiRBase ID
Paralogues Efe-Mir-2-o20  Efe-Mir-2-o21  Efe-Mir-2-o22  Efe-Mir-2-o23  Efe-Mir-2-P12f  Efe-Mir-2-P12g 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) E. fetida
Node of Origin (family) Protostomia
Genome context
(Efe_combined)		 Efet.01.159362: 648-708 [-]
Clustered miRNAs
(< 50kb from Mir-2-o24)		 Mir-2-o24 Efet.01.159362: 648-708 [-]
Mir-2-o23 Efet.01.159362: 1072-1126 [-]
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
UGAAAAGAAUUUCCAGAAAUUCCUCAUGGCACGGCAAAGUUGCGGUGAUGUGUAUGAUUGUCAAGUCAUAUCACAGCCUGCUUUGACGAGCCAUACACCAAGCACAGCAGAGNNNNNNNNN
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Structure
        10        20          30        40         50         
UGAAAAGAAUUUCCAGAAAUUCCUC--     A  G      U-| G        UAUGAU 
                           AUGGC CG CAAAGU  GC GUGAUGUG      U
                           UACCG GC GUUUCG  CG CACUAUAC      G
NNNNNNNNNGAGACGACACGAACCACA     A  A      UC^ A        UGAACU 
 .       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsNo
Tissue expression
- +
To
Star sequence

Efe-Mir-2-o24_5p*
mirBase accessionNone
Sequence 
0- ACGGCAAAGUUGCGGUGAUGUG -22
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Mature sequence

Efe-Mir-2-o24_3p
mirBase accessionNone
Sequence 
38- UAUCACAGCCUGCUUUGACGAGC -61
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