MirGeneDB 3.0

MirGeneDB ID

Agr-Mir-2-o86a

Family name MIR-2 (all species)
Species West Indian fuzzy chiton (Acanthopleura granulata )
MiRBase ID
Paralogues Agr-Mir-2-o85  Agr-Mir-2-o86a-v1  Agr-Mir-2-o86b  Agr-Mir-2-o87-v1  Agr-Mir-2-P12a  Agr-Mir-2-P12b  Agr-Mir-2-P12c  Agr-Mir-2-P12d  Agr-Mir-2-P12e  Agr-Mir-2-P12f1  Agr-Mir-2-P12f2 
Orthologues Gsp-Mir-2  Mom-Mir-2-o86  Ple-Mir-2 
Node of Origin (locus) A. granulata
Node of Origin (family) Protostomia
Genome context
(GCA_016165875.1_ASM1616587v1_Acanthopleura_granulata)
JABBOT010000080.1: 1400008-1400066 [+] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o86a-v2)
Mir-71 JABBOT010000080.1: 1396833-1396892 [+] Ensembl
Mir-2-o85 JABBOT010000080.1: 1397135-1397194 [+] Ensembl
Mir-2-P12a JABBOT010000080.1: 1397499-1397554 [+] Ensembl
Mir-2-o86a-v1 JABBOT010000080.1: 1400008-1400066 [+] Ensembl
Mir-2-o86a-v2 JABBOT010000080.1: 1400008-1400066 [+] Ensembl
Mir-2-o87-v1 JABBOT010000080.1: 1400233-1400291 [+] Ensembl
Mir-2-o87-v2 JABBOT010000080.1: 1400233-1400291 [+] Ensembl
Variant

Agr-Mir-2-o86a-v2

Seed AUCACAG
Precursor
(pre-Mir +30nt flank)
CACCCAAGUGAGUUCUGCUGAUACGGGGGCCCAUCAGGCCGACUGUGACAUGUGACAGUGGAAUCAUAUCACAGCCAGCUUUGAUGAGCCUUUGGUCAGAACAUACAGCUGGGUUUAGG
Get precursor sequence
Structure
        10           20        30         40        50        
CACCCAAGUGAGUUCUG---|    A       C      -  CGA      C   UGACA 
                    CUGAU CGGGGGC CAUCAG GC   CUGUGA AUG     G
                    GACUG GUUUCCG GUAGUU CG   GACACU UAC     U
GGAUUUGGGUCGACAUACAA^    -       A      U  ACC      A   UAAGG 
       110       100         90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Agr-Mir-2-o86a-v2_5p* (predicted)

mirBase accessionNone
Sequence
0- CCAUCAGGCCGACUGUGACAUG -22
Get sequence
Mature sequence

Agr-Mir-2-o86a-v2_3p

mirBase accessionNone
Sequence
36- UAUCACAGCCAGCUUUGAUGAGC -59
Get sequence
Variant

Agr-Mir-2-o86a-v1

Seed CACAGCC
Precursor
(pre-Mir +30nt flank)
CACCCAAGUGAGUUCUGCUGAUACGGGGGCCCAUCAGGCCGACUGUGACAUGUGACAGUGGAAUCAUAUCACAGCCAGCUUUGAUGAGCCUUUGGUCAGAACAUACAGCUGGGUUUAGG
Get precursor sequence
Structure
        10           20        30         40        50        
CACCCAAGUGAGUUCUG---|    A       C      -  CGA      CAUGUGACA 
                    CUGAU CGGGGGC CAUCAG GC   CUGUGA         G
                    GACUG GUUUCCG GUAGUU CG   GACACU         U
GGAUUUGGGUCGACAUACAA^    -       A      U  ACC      AUACUAAGG 
       110       100         90        80        70        60
Deep sequencing
Go to detailed chart
CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17), UGUG in loop
Tissue expression
- +
To
Star sequence

Agr-Mir-2-o86a-v1_5p* (predicted)

mirBase accessionNone
Sequence
0- CCAUCAGGCCGACUGUGACA -20
Get sequence
Mature sequence

Agr-Mir-2-o86a-v1_3p

mirBase accessionNone
Sequence
38- UCACAGCCAGCUUUGAUGAGC -59
Get sequence