Mir-2-o99

MirGeneDB ID

War-Mir-2-o99

Family name

MIR-2 (all species)

Species

Wirenia (Solenogaster) (Wirenia argentea)

MiRBase ID

Paralogues

War-Mir-2-o93 War-Mir-2-o94 War-Mir-2-o95 War-Mir-2-o96 War-Mir-2-o97 War-Mir-2-o98 War-Mir-2-P12

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

W. argentea

Node of Origin (family)

Protostomia

Genome context
(GCA_025802215.1_ASM2580221v1_Wirenia)

WNLI01082317.1: 2162-2221 [+] Ensembl

Clustered miRNAs
(< 50kb from Mir-2-o99)

Mir-2-o98 WNLI01082317.1: 1602-1658 [+] Ensembl
Mir-2-o99 WNLI01082317.1: 2162-2221 [+] Ensembl

Seed

AUCACAG

Precursor
(pre-Mir +30nt flank)

UGUCAGCGAUGCCAAUUUUGGUCUUUAUUCCCAUUCAAGUGACUGUGAUGUGUUCCUAUAGCAACCAUAUCACAGUCUGCUUUGAUGAGCAUAUGACUAAUAACACAUCACGUUUGACAA

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Structure

        10           20        30        40         50         
UGUCAGCGAUGCCAAUU---|     UU   UCC    C    -            UUCCUA 
                    UUGGUC  UAU   CAUU AAGU GACUGUGAUGUG      \
                    AAUCAG  AUA   GUAG UUCG CUGACACUAUAC      U
AACAGUUUGCACUACACAAU^     U-   CGA    U    U            CAACGA 
.       110       100         90        80        70        60

Deep sequencing

Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Unknown

Motifs

CNNC at 3p(+17)

Tissue expression

To

Star sequence

War-Mir-2-o99_5p* (predicted)

mirBase accession

None

Sequence

0- CCAUUCAAGUGACUGUGAUGUGU -23

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Mature sequence

War-Mir-2-o99_3p (predicted)

mirBase accession

None

Sequence

37- UAUCACAGUCUGCUUUGAUGAGC -60

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