MirGeneDB 3.0

MirGeneDB ID

Esc-Mir-12540

Family name MIR-12540 (all species)
Species Hawaiian bobtail squid (Euprymna scolopes)
MiRBase ID
Paralogues Esc-Mir-12540-v1 
Orthologues Obi-Mir-12540  Ovu-Mir-12540 
Node of Origin (locus) Coleoidea
Node of Origin (family) Coleoidea
Genome context
(esc-GCA_024364805.1_ASM2436480v1)
CM044527.1: 52798028-52798088 [+] Ensembl
Clustered miRNAs
(< 50kb from Mir-12540-v2)
Mir-12540-v1 CM044527.1: 52798028-52798088 [+] Ensembl
Mir-12540-v2 CM044527.1: 52798028-52798088 [+] Ensembl
Variant

Esc-Mir-12540-v2

Seed UCAAGAC
Precursor
(pre-Mir +30nt flank)
UUGUUCCUUUUAUUACAAUGUUGUGGGAGACCUUCAGCAUGGCCUUUGACAGUUUACACGACACUUCGCUAUCAAGACCAUGGUGAGAGUCUGCCGUAACACAGUACUCAACUGGUCAUAU
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Structure
        10          20        30        40        50        60
UUGUUCCUUUUAUUACAA--|       G    C    G     CC     C  UUUACAC 
                    UGUUGUGG AGAC UUCA CAUGG  UUUGA AG       G
                    ACAAUGCC UCUG GAGU GUACC  GAACU UC       A
UAUACUGGUCAACUCAUGAC^       G    A    G     A-     A  GCUUCAC 
 .       110       100        90        80         70
Deep sequencing
CommentAlthough the 3' uridine is templated this give a three 3-nt offset.
3' NTU Yes
MotifsCNNC at 3p(+17)
Star sequence

Esc-Mir-12540-v2_5p*

mirBase accessionNone
Sequence
0- CCUUCAGCAUGGCCUUUGACAG -22
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Mature sequence

Esc-Mir-12540-v2_3p

mirBase accessionNone
Sequence
40- AUCAAGACCAUGGUGAGAGUC -61
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Variant

Esc-Mir-12540-v1

Seed AUCAAGA
Precursor
(pre-Mir +30nt flank)
UUGUUCCUUUUAUUACAAUGUUGUGGGAGACCUUCAGCAUGGCCUUUGACAGUUUACACGACACUUCGCUAUCAAGACCAUGGUGAGAGUCUGCCGUAACACAGUACUCAACUGGUCAUAU
Get precursor sequence
Structure
        10          20        30        40        50        60
UUGUUCCUUUUAUUACAA--|       G    C    G     CC     C   UUACAC 
                    UGUUGUGG AGAC UUCA CAUGG  UUUGA AGU      G
                    ACAAUGCC UCUG GAGU GUACC  GAACU UCG      A
UAUACUGGUCAACUCAUGAC^       G    A    G     A-     A   CUUCAC 
 .       110       100        90        80         70
Deep sequencing
CommentAlthough the 3' uridine is templated this give a three 3-nt offset.
3' NTU Yes
MotifsCNNC at 3p(+17)
Star sequence

Esc-Mir-12540-v1_5p*

mirBase accessionNone
Sequence
0- CCUUCAGCAUGGCCUUUGACAGU -23
Get sequence
Mature sequence

Esc-Mir-12540-v1_3p

mirBase accessionNone
Sequence
39- UAUCAAGACCAUGGUGAGAGUC -61
Get sequence