MirGeneDB 3.0
MirGeneDB ID

Cin-Let-7-P2o1

Family name LET-7 (all species)
Species Sea Squirt (Ciona intestinalis)
MiRBase ID cin-let-7a-1
Paralogues Cin-Let-7-P1  Cin-Let-7-P2o1-v1  Cin-Let-7-P2o2  Cin-Let-7-P2o3  Cin-Let-7-P2o4  Cin-Let-7-P2o5 
Orthologues Aae-Let-7  Aga-Let-7  Agr-Let-7  Bge-Let-7  Bko-Let-7  Bpl-Let-7  Cte-Let-7  Dan-Let-7  Dlo-Let-7  Dma-Let-7  Dme-Let-7  Dmo-Let-7  Dpu-Let-7  Dsi-Let-7  Dya-Let-7  Eba-Let-7  Egr-Let-7  Esc-Let-7  Gpa-Let-7  Gsa-Let-7  Gsp-Let-7  Hme-Let-7  Hmi-Let-7  Hru-Let-7  Isc-Let-7  Lan-Let-7  Lgi-Let-7  Lhy-Let-7  Llo-Let-7  Mgi-Let-7  Mom-Let-7  Npo-Let-7  Obi-Let-7  Ofu-Let-7  Ovu-Let-7  Pau-Let-7  Pca-Let-7  Pcr-Let-7  Pdu-Let-7  Pfl-Let-7  Ple-Let-7  Pmi-Let-7  Pve-Let-7  Rph-Let-7  Sko-Let-7  Sma-Let-7  Sne-Let-7  Snu-Let-7  Spu-Let-7  Sro-Let-7  Tca-Let-7  Tur-Let-7  War-Let-7  Xbo-Let-7 
Node of Origin (locus) C. intestinalis
Node of Origin (family) Bilateria
Genome context
(ci3)		 4: 2157421-2157502 [+] UCSC Ensembl
Clustered miRNAs
(< 50kb from Let-7-P2o1-v2)		 Let-7-P2o1-v2 4: 2157421-2157502 [+] UCSC Ensembl
Let-7-P2o1-v1 4: 2157422-2157502 [+] UCSC Ensembl
Let-7-P2o2 4: 2157552-2157624 [+] UCSC Ensembl
Let-7-P2o3 4: 2157948-2158018 [+] UCSC Ensembl
Let-7-P2o4 4: 2158141-2158206 [+] UCSC Ensembl
Let-7-P2o5 4: 2158340-2158414 [+] UCSC Ensembl
Variant

Cin-Let-7-P2o1-v2
Seed UGAGGUA
Precursor
(pre-Mir +30nt flank)		 
GACCACCGAAACAACAUGGCCGUGACCACAAUGAGGUAGUAGGUUAUGCAGUUAUAGCCUCCCUUUUAUACUGAGUGGAGCACAGGAGAUACUGUGUAACCUUCUGCCUUUGUGGCUUCGUGUUUCUAUCAUCAGCUCCCAC
Get precursor sequence
Structure
        10         20         30        40        50        60        70 
GACCACCGAAACAACAU-   -| UGA     AU       U            UAUAGCCUCCCUUUUAUA 
                  GGC CG   CCACA  GAGGUAG AGGUUAUGCAGU                  C
                  UUG GC   GGUGU  UUCCGUC UCCAAUGUGUCA                  U
CACCCUCGACUACUAUCU   U^ UUC     --       U            UAGAGGACACGAGGUGAG 
140       130       120         110       100        90        80
Deep sequencing
Go to detailed chart
CommentThis paralogue is related to the vertebrate P2s but it is not yet possible to disentangle the phylogenetic history of vertebrate Let-7 P2s and thus the ascidian genes are classified as orphans pending further data. There is also a second Dicer cut -1 on the 3p arm.
3' NTU Yes
MotifsUG at 5p(-14), CNNC at 3p(+17)
Tissue expression
- +
C- C- C- C- C- C- C- C- C- C- Ga La La
Mature sequence

Cin-Let-7-P2o1-v2_5p
mirBase accessionMIMAT0006086
Sequence 
0- AUGAGGUAGUAGGUUAUGCAGU -22
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Star sequence

Cin-Let-7-P2o1-v2_3p*
mirBase accessionMIMAT0015247
Sequence 
62- UGUGUAACCUUCUGCCUUUG -82
Get sequence
Variant

Cin-Let-7-P2o1-v1
Seed GAGGUAG
Precursor
(pre-Mir +30nt flank)		 
ACCACCGAAACAACAUGGCCGUGACCACAAUGAGGUAGUAGGUUAUGCAGUUAUAGCCUCCCUUUUAUACUGAGUGGAGCACAGGAGAUACUGUGUAACCUUCUGCCUUUGUGGCUUCGUGUUUCUAUCAUCAGCUCCCAC
Get precursor sequence
Structure
        10           20        30        40        50        60        70
ACCACCGAAACAACAUG--  -| UGA     AU       U            UAUAGCCUCCCUUUUAUA 
                   GC CG   CCACA  GAGGUAG AGGUUAUGCAGU                  C
                   UG GC   GGUGU  UUCCGUC UCCAAUGUGUCA                  U
CACCCUCGACUACUAUCUU  U^ UUC     --       U            UAGAGGACACGAGGUGAG 
 .       130       120       110         100        90        80
Deep sequencing
Go to detailed chart
CommentThis paralogue is related to the vertebrate P2s but it is not yet possible to disentangle the phylogenetic history of vertebrate Let-7 P2s and thus the ascidian genes are classified as orphans pending further data. There is also a second Dicer cut -1 on the 3p arm.
3' NTU Yes
MotifsCNNC at 3p(+17)
Tissue expression
- +
C- C- C- C- C- C- C- C- C- C- Ga La La
Mature sequence

Cin-Let-7-P2o1-v1_5p
mirBase accessionMIMAT0006086
Sequence 
0- UGAGGUAGUAGGUUAUGCAGUU -22
Get sequence
Star sequence

Cin-Let-7-P2o1-v1_3p*
mirBase accessionMIMAT0015247
Sequence 
60- CUGUGUAACCUUCUGCCUUUG -81
Get sequence