Mir-2-o160

MirGeneDB ID

Pcr-Mir-2-o160

Family name

MIR-2 (all species)

Species

Tiger flatworm (Prostheceraeus crozieri )

MiRBase ID

Paralogues

Pcr-Mir-2-o159

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

P. crozeri

Node of Origin (family)

Protostomia

Genome context
(GCA_907163375.1_PROCRO_GENOME_genomic)

CAJQUW010002633.1: 636207-636265 [+] Ensembl

Clustered miRNAs
(< 50kb from Mir-2-o160)

Mir-71-v1 CAJQUW010002633.1: 635983-636044 [+] Ensembl
Mir-71-v2 CAJQUW010002633.1: 635985-636042 [+] Ensembl
Mir-2-o159 CAJQUW010002633.1: 636086-636150 [+] Ensembl
Mir-2-o160 CAJQUW010002633.1: 636207-636265 [+] Ensembl

Seed

UUCCAAU

Precursor
(pre-Mir +30nt flank)

UCCUUUUUAGAUUAUACGUGUUUUUCCUUUAUUCCAAUUAGGCUGUGUUGCUUUGAAGUUAACUAGUAUCACAGCCCUGCUUGGAAUAAUGAGAUGUCACUGUGCUGCCGGAAGAUUCU

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Structure

        10          20         30        40         50        
UCCUUUUUAGAUUAUAC--   UU-|   CU         U    -     U    UUGAA 
                   GUG   UUUC  UUAUUCCAA UAGG CUGUG UGCU     G
                   CAC   AGAG  AAUAAGGUU GUCC GACAC AUGA     U
UCUUAGAAGGCCGUCGUGU   UGU^   U-         C    C     U    UCAAU 
       110       100        90         80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

To

Mature sequence

Pcr-Mir-2-o160_5p

mirBase accession

None

Sequence

0- AUUCCAAUUAGGCUGUGUUGCU -22

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Co-mature sequence

Pcr-Mir-2-o160_3p

mirBase accession

None

Sequence

36- UAUCACAGCCCUGCUUGGAAUAA -59

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