Mir-2-o159

MirGeneDB ID

Pcr-Mir-2-o159

Family name

MIR-2 (all species)

Species

Tiger flatworm (Prostheceraeus crozieri )

MiRBase ID

Paralogues

Pcr-Mir-2-o160

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

P. crozeri

Node of Origin (family)

Protostomia

Genome context
(GCA_907163375.1_PROCRO_GENOME_genomic)

CAJQUW010002633.1: 636086-636150 [+] Ensembl

Clustered miRNAs
(< 50kb from Mir-2-o159)

Mir-71-v1 CAJQUW010002633.1: 635983-636044 [+] Ensembl
Mir-71-v2 CAJQUW010002633.1: 635985-636042 [+] Ensembl
Mir-2-o159 CAJQUW010002633.1: 636086-636150 [+] Ensembl
Mir-2-o160 CAJQUW010002633.1: 636207-636265 [+] Ensembl

Seed

CACAGCC

Precursor
(pre-Mir +30nt flank)

CGGUCGUCUAUCAUCAUACCAUCUCGUUGGUCGUCAAUAUUGUCUGUCGGUAGUGAUUCAAUGUAAAAACUUAUCACAGCCAGUAUUGAUGAGCCAGUUGGAAGGUUUGAAGCCACACUUUUCCU

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Structure

        10          20        30         40        50        60  
CGGUCGUCUAUCAUCAU--   A   CG    -|           U    C    GUGAUUCAA 
                   ACC UCU  UUGG UCGUCAAUAUUG CUGU GGUA         \
                   UGG AGG  GACC AGUAGUUAUGAC GACA CUAU         U
UCCUUUUCACACCGAAGUU   A   UU    G^           C    -    UCAAAAAUG 
   120       110       100        90        80         70

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17), UGUG in loop

Tissue expression

To

Star sequence

Pcr-Mir-2-o159_5p*

mirBase accession

None

Sequence

0- UCGUCAAUAUUGUCUGUCGGUA -22

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Mature sequence

Pcr-Mir-2-o159_3p

mirBase accession

None

Sequence

43- UCACAGCCAGUAUUGAUGAGCC -65

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