Mir-279-o1

MirGeneDB ID

Dpu-Mir-279-o1

Family name

MIR-279 (all species)

Seed

GACUAGA

Species

Common water flea (Daphnia pulex)

MiRBase ID

Paralogues

Dpu-Mir-279-o2 Dpu-Mir-279-o3 Dpu-Mir-279-o4 Dpu-Mir-279-o5 Dpu-Mir-279-o6

Orthologues

Aae-Mir-279-P1 Bpl-Mir-279 Cgi-Mir-279 Dan-Mir-279-P1 Dma-Mir-279-o1 Dme-Mir-279-P1 Dmo-Mir-279-P1 Dsi-Mir-279-P1 Dya-Mir-279-P1 Esc-Mir-279 Isc-Mir-279 Lgi-Mir-279 Npo-Mir-279 Obi-Mir-279 Ovu-Mir-279

Node of Origin (locus)

Daphnia

Node of Origin (family)

Protostomia

Genome context
(Daphnia_pulex.V1.0)

DAPPUscaffold_32: 69703-69769 [-] Ensembl

Clustered miRNAs
(< 50kb from Mir-279-o1)

Mir-965 DAPPUscaffold_32: 27782-27843 [-] Ensembl
Mir-279-o2 DAPPUscaffold_32: 68663-68726 [-] Ensembl

Precursor
(pre-Mir +30nt flank)

AGUCGACUCAAAACUCGGUCGGAUUUCGUCGAUGAGUGGGGAUCGUUAGUCGCGUUGAAAUUUCCUCAUGUUCGUGACUAGAUCUGACACUCGUCCACGACGUCAACCAAAAAUCACCCCAUGUGAG

Get precursor sequence

Structure

        10        20          30         40        50        60   
AGUCGACUCAAAACUCGGUCG--   U    C        G-|    GU        UUGAAAUU 
                       GAU UCGU GAUGAGUG  GGAUC  UAGUCGCG        U
                       CUG AGCA CUGCUCAC  UCUAG  AUCAGUGC        C
GAGUGUACCCCACUAAAAACCAA   C    C        AG^    --        UUGUACUC 
     120       110       100        90          80        70

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-279 genes were present in the last common ancestor of protostomes and how the Drosophila Mir-279s relate to the other invertebrate Mir-279s and thus these multiple paralogues in invertebrates are classified here as orphans pending new data.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

To

Star sequence

Dpu-Mir-279-o1_5p* (predicted)

mirBase accession

None

Sequence

0- GAUGAGUGGGGAUCGUUAGUCGCG -24

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Mature sequence

Dpu-Mir-279-o1_3p

mirBase accession

None

Sequence

44- UGACUAGAUCUGACACUCGUCCA -67

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