Mir-2-o30

MirGeneDB ID

Cgi-Mir-2-o30

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Pacific oyster (Crassostrea gigas)

MiRBase ID

Paralogues

Cgi-Mir-2-o28 Cgi-Mir-2-o29 Cgi-Mir-2-o31a Cgi-Mir-2-o31b Cgi-Mir-2-o32 Cgi-Mir-2-o33 Cgi-Mir-2-o34 Cgi-Mir-2-o35

Orthologues

Node of Origin (locus)

C. gigas

Node of Origin (family)

Protostomia

Genome context
(CGI_GCA_000297895.1)

scaffold602: 445414-445474 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

CAAUAUUUUUGGAAGUUUGAAAAAGUGUAGCCAUCAAAGUGACUGUGAAAUGUUGAAUUAAAAUUCAUAUCACAGCCAGCUUUGAUGAGCUUCAUGUUUCUGUCUGACACCAUUUCAGUCU

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Structure

        10           20        30         40         50         
CAAUAUUUUUGGAAGUUU---    AA   U   -         GA-|     A   UUGAAU 
                     GAAA  GUG AGC CAUCAAAGU   CUGUGA AUG      \
                     CUUU  UAC UCG GUAGUUUCG   GACACU UAC      U
UCUGACUUUACCACAGUCUGU    G-   U   A         ACC^     A   UUAAAA 
 .       110       100         90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

A-	D-	D-	Eg	F-	Gi	He	Ju	L-	Ma	Ma	Pe	Re	Sp	Um	Ea	Fe	Ha	La	Ma

Star sequence

Cgi-Mir-2-o30_5p*

mirBase accession

None

Sequence

0- CCAUCAAAGUGACUGUGAAAUG -22

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Mature sequence

Cgi-Mir-2-o30_3p

mirBase accession

None

Sequence

37- UAUCACAGCCAGCUUUGAUGAGCU -61

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